RealHelix™ Premier qPCR Kit [Green] is designed to perform a rapid, highly specific, and sensitive real-time quantification of target DNA. Specially designed buffer and antibody-mediated hot start enzyme considerably reduce the primer dimer formations and ensure producing reliable data. The convenient 2x concentrated premix contains Ab+Taq polymerase, dNTPs, buffers, Mg²⁺, a green fluorescent dye, ROX passive dye, and stabilizing agent. The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiple reactions.

Figure 1. Sensitive detection using RealHelix™ Premier qPCR Kit [Green, Low ROX].

A standard sample of 10-fold serial-diluted genomic DNA was analyzed using the RealHelix™ Premier qPCR Kit for the human glucose-6-phosphate dehydrogenase gene-specific primer set. The accuracy of real-time PCR using the RealHelix™ Premier qPCR Kit is demonstrated by R² value=0.99887. NTC: Negative control.

Figure 2. Amplification of target DNA with various G+C contents using RealHelix™ Premier qPCR Kit [Green, Low ROX].

It was evaluated by real-time PCR using 10-fold serial diluted human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, and blue: 0.5 ng/rxn) with each instrument. NTC: Negative control.

Figure 3. Comparison of real-time PCR using RealHelix™ Premier qPCR Kit [Green, Low ROX] with other company's related products.

10-fold serial dilutions of the human genomic DNA (red: 50 ng/rxn, green: 5 ng/rxn, and blue: 0.5 ng/rxn) from whole blood were analyzed using the human HPRT-1 gene-specific primer set. Reactions were run on a real-time PCR instrument, CFX-96 (Bio-Rad, USA). NTC: Negative control.

Storage

• Store below -20 ℃
• Should be stored in a dark place.

Shelf life

• 12 months